FluoroTectâ„¢ GreenLys in vitro Translation Labeling System
Fluorescent Labeling and Detection of in Vitro Synthesized Proteins
- Data in minutes versus overnight exposures associated with radioactivity-based systems
- "In-gel" detection; No need to transfer, fix or dry gels
- No radioactivity required
Catalog Number:
Size
Catalog Number: L5001
This system enables fluorescent labeling and detection of in vitro synthesized proteins, based on a lysine-charged tRNA labeled with the fluorophore BODIPY®-FL at the ε position. Fluorescent lysine residues are incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity.
Detection of the labeled proteins is accomplished in 2–5 minutes directly "in-gel" by use of a laser-based fluorescent gel scanner. This eliminates any need for protein gel manipulation such as fixing/drying or any safety, regulatory and waste disposal issues associated with the use of radioactively labeled amino acids. The convenience of "in-gel" detection also avoids the time-consuming electroblotting and detection steps of conventional non-isotopic systems.
The modified charged tRNA can be used with a variety of Promega translation systems including: Rabbit Reticulocyte Lysate, TnT® Coupled Transcription/Translation System, Wheat Germ Extract and E. coli S30 Extract.
For more information, see the Protocols & Applications Guide.
Schematic illustrating the incorporation of FluoroTect™ GreenLys-labeled lysine into nascent protein.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
FluoroTectâ„¢ GreenLys tRNA |
L500A | 1 × 40μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
FluoroTect™ GreenLys incorporates Fluorotag® technology, which is licensed under U.S. Pat. Nos. 6,306,628 and 7,252,932. Commercial use of this product requires a license from AmberGen, Inc. Fluorotag is a registered trademark of AmberGen, Inc.
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