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L5620_pCMVTNT--Vector--20ug_3 L5610_pTNT--Vector--20ug_3

Convenient Expression of Cloned Genes In Vitro or In Vivo

  • Express cloned genes in vitro or in vivo from SP6- or T7-based coupled in vitro transcription/translation systems

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Catalog Number: L5620

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Catalog Number: L5610

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Overview
Protocols
Specifications
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Express Cloned Genes In Vitro or In Vivo

The pCMVTnT™ and pTnT™ Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems. The presence of RNA phage promoters also allows highly efficient synthesis of RNA in vitro. Both vectors contain a 5´ β-globin leader sequence, and the pTnT™ vector also contains a synthetic poly(A)30 tail; these elements have been shown to enhance expression of certain genes. The multiple cloning site provides a selection of restriction sites.

For in vivo expression, the pCMVTnT™ Vector contains a CMV enhancer/promoter region, which allows strong constitutive expression in many cell types.

3602MA-W
pTnT™ Vector GenBank® Accession Number AF479322 and vector sequence text file.
3603MA-W

pCMVTnT™ Vector GenBank® Accession Number AF477200 and vector sequence text file

Specifications

Catalog Number:

What's in the box?

Item Part # Size

pCMVTnT™ Vector

L562A 1 × 20μg

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG

What's in the box?

Item Part # Size

pTnT™ Vector

L561A 1 × 20μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG

Resources

No related resources available