Activity of Cloning Enzymes in Buffer H
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EcoRI and PstI are commonly used enzymes in double digests and cloning applications. Their optimal buffer is H. This reference table provides the relative activity of other cloning enzymes in Buffer H for double digest purposes.
| ApaI | <10% | NheI | 10–25% |
| BamHI |
50–75% |
NotI | 100–125%* |
| BclI |
50–75% |
PstI | 100% |
| BglI | 75–100% |
SacI | 25–50% |
| ClaI |
50–75% |
SacII | >125%* |
| EcoRI |
100% |
SalI | 25–50% |
| EcoRV | 50–75% |
SmaI | <10% |
| HindIII |
25–50% |
SpeI | 25–50% |
| KpnI | <10% | SphI | >125%* |
| MluI |
100–125%* |
XbaI | 100–125%* |
| NcoI | 100–125%* |
XhoI | 100–125%* |
*Unit activity is based on recommended buffer. In Buffer H, some enzymes have enhanced activity.