Composition of Promega Restriction Enzyme Reaction Buffers 1X
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Promega Restriction Enzyme Reaction Buffers are provided as 10X stock solutions. This reference table provides compositions for Restriction Enzyme Reaction Buffers, listed as 1X concentrations.
| Buffer | pH (at 37°C) |
Tris-HCl (mM) |
MgCl2 (mM) |
NaCl (mM) |
KCl (mM) |
DTT (mM) |
|---|---|---|---|---|---|---|
| A | 7.5 | 6 | 6 | 6 | – | 1 |
| B | 7.5 | 6 | 6 | 50 | – | 1 |
| C | 7.9 | 10 | 10 | 50 | – | 1 |
| D | 7.9 | 6 | 6 | 150 | – | 1 |
| E | 7.5 | 6 | 6 | 100 | – | 1 |
| F | 8.5 | 10 | 10 | 100 | – | 1 |
| G | 8.2 | 50 | 5 | – | – | – |
| H | 7.5 | 90 | 10 | 50 | – | – |
| J | 7.5 | 10 | 7 | – | 50 | 1 |
| K | 7.4 | 10 | 10 | – | 150 | – |
| L | 9.0 | 10 | 3 | 100 | – | – |
MULTI-CORE™ Buffer (1X) = 25mM Tris-Acetate, pH 7.5 (at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.
Notes:
- For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
- For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.