Relative Activity of Restriction Enzymes in Promega 10X Buffers
For ordering information on the products discussed here, please visit our Restriction Enzymes product listing.
The 10X Reaction Buffer supplied with each restriction enzyme is optimized to give 100% activity. In many cases, good activity is also obtained using one of the 4-CORE® 10X Buffers (Cat.# R9921). This reference table may be used to select the best buffer for digestions with multiple restriction enzymes. Enzyme activity is expressed as a percent of the activity obtained with the optimized buffer for each enzyme.
| Promega Enzyme | Buffer Supplied with Enzyme | 4 CORE® Buffers | MULTI-CORE™ Buffer | Enzyme Assay Temperature | |||
|---|---|---|---|---|---|---|---|
| A | B | C | D | ||||
| AgeI | K | 25–50% | 25–50% | 25–50% | 50–75% | 100% | 37°C |
| AluI | B | 75–100% | 100% | 75–100% | 10–25% | 10–25% | 37°C |
| ApaI | A | 100% | 50–75% | 50–75% | <10% | 75–100% | 37°C |
| BamHI | E | 75–100% ** | 75–100% | 75–100% | 50–75% | 75–100% | 37°C |
| BclI | C | 10–25% | 75–100% | 100% | 50–75% | 10–25% | 50°C |
| BglI | D | 10–25% | 25–% | 75–100% | 100% | 100% | 37°C |
| BglII | D | 25–50% | 75–100% | 75–100% | 100% | <10% | 37°C |
| CfoI | B | 75–100% | 100% | 75–100% | 25–50% | 100% | 37°C |
| ClaI | C | 75–100% | 75–100% | 100% | 75–100% | 100% | 37°C |
| DdeI | D | 25–50% | 25–50% | 50–75% | 100% | 25–50% | 37°C |
| DpnI | B | 50–75% | 100% | 75–100% | 50–75% | 100% | 37°C |
| EcoRI | H | 25–50% | 50–75% | 50–75% | 50–75% | 100%** | 37°C |
| EcoRV | D | 10–25% | 25–50% | 50–75% | 100% | 100% | 37°C |
| HaeIII | C | 75–100% | 75–100% | 100% | 50–75% | 100% | 37°C |
| HhaI | C | 50–75% | 75–100% | 100% | 50–75% | 75–100% | 37°C |
| HindIII | E | 25–50% | 100% | 75–100% | 10–25% | 50–75% | 37°C |
| HinfI | B | 50–75% | 100% | 75–100% | 75–100% | 50–75% | 37°C |
| HpaII | A | 100% | 50–75% | 50–75% | 10–25% | 100% | 37°C |
| I-PpoI | I-PpoI | 10–25% | 25–50% | 25–50% | 25–50% | — | 37°C |
| KpnI | J | 100%** | 25–50% | 25–50% | <10% | 75–100% | 37°C |
| MboI | C | 10–25% | 75–100% | 100% | 50–75% | <10% | 37°C |
| MluI | D | 10–25% | 25–50% | 50–75% | 100% | 10–25% | 37°C |
| MspI | B | 75–100% | 100% | 75–100% | 25–50% | 25–50% | 37°C |
| NcoI | D | 50–75% | 75–100% | 75–100% | 100% | 75–100% | 37°C |
| NdeI | D | <10% | <10% | 25–50% | 100% | 25–50% | 37°C |
| NheI | B | 75–100% | 100% | 75–100% | 10–25% | 100% | 37°C |
| NotI | D | <10% | 10–25% | 25–50% | 100% | 25–50% | 37°C |
| PstI | H | 10–25% | 50–75% | 50–75% | 50–75% | 25–50% | 37°C |
| PvuI | D | 10–25% | 25–50% | 50–75% | 100% | <10% | 37°C |
| RsaI | C | 75–100% | 75–100% | 100% | <10% | <10% | 37°C |
| SacI | J | 75–100% | 25–50% | 25–50% | <10% | 100% | 37°C |
| SacII | C | 100%* | 50–75% | 100% | 50–75% | <10% | 37°C |
| SalI | D | <10% | 10–25% | 25–50% | 100% | <10% | 37°C |
| ScaI | K | <10% | 100%** | 50–75% | 75–100% | 10–25% | 37°C |
| SgfI | C | 25–50% | 25–50% | 100% | <10% | <10% | 37°C |
| SmaI | J | <10% | <10% | 10% | <10% | 100% | 25°C |
| SpeI | B | 75–100% | 100% | 75–100% | 75–100% | 100% | 37°C |
| SphI | K | 75–100% | 75–100%** | 100% | 75–100% | 10–25% | 37°C |
| TaqI | E | 10–25% | 25–50% | 50–75% | 50–75% | 100% | 65°C |
| XbaI | D | 50–75% | 75–100% | 75–100% | 100% | 100% | 37°C |
| XhoI | D | 25–50% | 75–100% | 75–100% | 100% | 10–25% | 37°C |