HaloTag® Biotin Ligand Protocol
Instructions for Use of Product(s)
G8281, G8282
Literature # 9PIG828
The HaloTag® Interchangeable Labeling Technology is a novel tool for imaging live or fixed mammalian cells that express the HaloTag® Protein or Protein Fusion, analyzing post-translational modification of labeled fusion proteins, and isolating proteins and protein complexes. HaloTag® Protein is encoded by the a variety of HaloTag® Vectors, which are designed to allow protein fusions. The HaloTag® Biotin Ligand is a small chemical tag that readily crosses the cell membrane and comprises the HaloTag® Reactive Linker and biotin. The HaloTag® Ligands have shown no detectable toxicity or morphological side effects at the recommended labeling conditions in cell lines tested (HeLa, CHO-K1). The HaloTag® Biotin Ligand may be used as an affinity tag to capture a protein of interest using the strong biotin-streptavidin interaction.
Printed in USA. Revised 5/24.