Pfu DNA Polymerase Protocol
Instructions for Use of Product(s)
M7741, M7745
Literature # 9PIM774
Pfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90,000 daltons as estimated from the predicted amino acid sequence and exhibits 3´→5´ exonuclease (proofreading) activity. Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity.
Summary of Changes
The following changes were made to the 4/24 revision of this document:
1. Signature was updated.
2. Contents moved to latest template and font.
Printed in USA. Revised 4/24.