cAMP-Glo™ Max Assay Technical Manual
Instructions for Use of Product(s)
V1681, V1682, V1683
Literature # TM347
The cAMP-Glo™ Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate G protein-coupled receptors (GPCRs) coupled with adenylate cyclase typically alter intracellular cAMP levels. The cAMP-Glo™ Max Assay monitors cAMP levels in cells in response to the effect of agonists, antagonists or test compounds on GPCRs.
The cAMP-Glo™ Max Assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity; this stimulation depletes ATP in the reaction resulting in decreased light production in a coupled luciferase reaction.
Summary of Changes
The following changes were made to the 7/19 revision of this document:
1. Corrected the volume in Section 5.D for Assay Protocol Using a 4:1 Ratio, Step 4.
2. Corrected reference to Table 4 for volume of cAMP Detection Solution in Sections 5.B, 5.C and 5.D.
3. Updated Related Products.
Printed in USA. Revised 7/19.