T Cell Activation Bioassay (NFAT), Propagation Model, Technical Manual
Instructions for Use of Product(s)
J1601
Literature # TM489
The T Cell Activation Bioassay (NFAT), Propagation Model, is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used for the discovery and development of novel biologic and cell therapies aimed at inducing, strengthening and/or engineering T cell responses. The assay consists of a genetically engineered Jurkat T cell line that expresses a luciferase reporter driven by an NFAT-response element (NFAT-RE). The TCR/CD3 Effector Cells (NFAT) are provided in Cell Propagation Model (CPM) format, which includes cryopreserved cells that can be thawed, propagated and banked for long-term use.
The TCR/CD3 Effector Cells express endogenous TCR, CD3 and CD28 receptors. When the TCR/CD3 Effector Cells (NFAT) are engaged with an appropriate TCR/CD3 ligand or anti-TCR/CD3 antibody, the TCR transduces intracellular signals resulting in NFAT-RE-mediated luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System and a standard luminometer, such as the GloMax® Discover System.
Summary of Changes
The following changes were made to the 6/25 revision of this document:
1. Removed an expired patent statement and updated another patent statement.
2. Updated fonts and the cover image.
3. Revised numbering in Section 4.D was due to a missing number.
4. Made miscellaneous minor text edits.
5. Added two third party trademark attributions.
Revised 6/25.