GenePrint® Matrix Standards for Use on the Spectrum Compact CE System Technical Manual
Instructions for Use of Product(s)
B1930, DG4800
Literature # TM594
Proper generation of spatial and spectral calibration files is critical to evaluate multicolor STR systems with the Spectrum Compact CE System. Refer to the Spectrum Compact CE System Operating Manual #TMD058 for the instrument maintenance schedule and instructions for installation of the capillary array, buffers and polymer cartridge and performing spatial calibration.
The GenePrint® 5C Matrix Standards consist of DNA fragments labeled with different fluorescent dyes (fluorescein, JOE, TMR-ET, CXR-ET and WEN) in one tube, and the PowerPlex®
4C Matrix Standards consist of DNA fragments labeled with four different fluorescent dyes (Fluorescein, JOE, TMR and CXR) in one tube. Once generated, the spectral calibration file is applied during sample detection to calculate the spectral overlap and separate the raw fluorescent signals into individual color signals. A spectral calibration must be generated for each individual instrument, and must be performed after the installation of a capillary array following a spatial calibration. A spectral calibration should also be performed after any major maintenance on the system, such as changing the laser, replacing the camera or if a decrease in spectral separation is observed in the STR results. We also recommend generating a new matrix any time the instrument is moved to a new location.
Summary of Changes
The following changes were made to the 12/21 revision of this document:
1. Updated Figures 24 and 25.
2. Updated disclaimers.
Revised 12/21.