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GoTaq® Enviro PMMoV Quant Kit, Quasar® 670 Technical Manual

Instructions for Use of Product(s)
AM2140

Literature # TM667

The GoTaq® Enviro PMMoV Quant Kit, Quasar® 670, can be used for identifying human fecal contamination in a sample, process control for sample preparation, RT-qPCR analysis of wastewater and other relevant environmental samples, and normalizing other nucleic acid targets (e.g., SARS-CoV-2 RNA in wastewater). PMMoV (Pepper Mild Mottle Virus) is a single-stranded RNA virus infecting peppers. Due to the nature of the human diet, the virus and its genetic signature are present in human feces, acting as a human fecal indicator. PMMoV is widely distributed and commonly found in wastewater, surface water and other environmental water sources.

Summary of Changes
The following changes were made to the 4/24 revision of this document:
1. Added Section 7.B, Troubleshooting.

Revised 4/24.