IL-12 Bioassay, Propagation Model Technical Manual
Instructions for Use of Product(s)
J3042
Literature # TM588
The IL-12 Bioassay, Propagation Model is a bioluminescent cell-based assay designed to measure IL-12 stimulation or inhibition. The IL-12 Bioassay Cells are provided in a Cell Propagation Model (CPM) format, as cryopreserved cells that can be thawed, propagated and banked for long-term use. This genetically engineered human cell line expresses a luciferase reporter driven by a response element (RE). When IL-12 binds to IL-12R it transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System and a standard luminometer, such as the GloMax® Discover System.
Summary of Changes
The following changes were made to the 5/26 revision of this document:
1. Removed Section 9.B, Related Products.
2. Made minor text and formatting edits.
Revised 5/26.