Lumit® dsRNA Detection Assay Technical Manual
Instructions for Use of Product(s)
W2041, W2042
Literature # TM746
Traditional methods for quantifying dsRNA, such as dot blot or enzyme-linked immunosorbent assay (ELISA), rely on antibody-based detection. However, these methods are often limited by sequence bias, reduced sensitivity and labor intensive protocols.
In contrast, the Lumit® dsRNA Detection Assay is a homogeneous, bioluminescent approach for dsRNA quantification, eliminating the need for antibodies or wash steps. This assay uses NanoLuc® Binary Technology (NanoBiT), a split luciferase complementation system specifically designed for biomolecular interaction studies. NanoBiT comprises two subunits: Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acids), both of which are engineered for stability and minimal spontaneous association.
In the Lumit® dsRNA Detection Assay, the sample containing dsRNA is incubated with two dsRNA-binding domains, one labeled with LgBiT and the other with SmBiT. When these binding domains interact with dsRNA, the LgBiT and SmBiT subunits are brought into proximity, reconstituting the NanoBiT® enzyme and producing luminescence in the presence of the Lumit® substrate. The resulting luminescence is directly proportional to the dsRNA concentration in the sample, enabling rapid and accurate quantification without wash steps.
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