Maxwell® RSC Enviro Total Nucleic Acid Kit Technical Manual
Instructions for Use of Product(s)
AS1831
Literature # TM663
The Maxwell® RSC Enviro Total Nucleic Acid Kit is a convenient method to directly capture and concentrate total nucleic acid (TNA) from a large volume of water using PureYield™ columns. The method uses a short protocol that minimizes the need for specialized laboratory equipment. In a first step total nucleic acid from a large-volume sample (e.g., 40ml of wastewater) is captured on a PureYield™ Binding Column and then eluted in 0.5ml. In a second step the material is purified and concentrated using the Maxwell® RSC System for walk-away-automation with fast, reliable total nucleic acid purification. This method achieves high yields, consistent recovery and a significant reduction in PCR inhibitors.
The total nucleic acid extracted using the Maxwell® RSC Enviro Total Nucleic Acid Kit can be analyzed for SARS-CoV-2 targets using a SARS-CoV-2 RT-qPCR kit for wastewater.
Summary of Changes
The following changes were made to the 1/23 version of this document:
1. Revised Sections 1, 3, 4 and 8.D.
2. Added new Section 8.C.
3. Updated document font.
Revised 1/23.