NanoLuc® Labeling Kit Technical Manual
Instructions for Use of Product(s)
VB1500
Literature # TM666
NanoLuc® luciferase is a small (19.1kDa), stable reporter enzyme that generates a bright luminescence signal. The small size and bright signal make NanoLuc® luciferase a versatile reporter that is used in many protein analysis applications including NanoBRET™ target engagement and protein interaction applications. Due to its unique properties, NanoLuc® luciferase is useful as a reporter in enzyme linked immunosorbent assays (ELISA). The NanoLuc® Labeling Kit enables labeling of antibodies and other proteins (e.g., streptavidin) with NanoLuc® luciferase for the development of bioluminescent signal-based ELISA.
In this kit, HaloTag® technology is used to label antibodies with NanoLuc® luciferase. HaloTag is a fusion protein that covalently binds its ligand under physiological conditions and has been used in variety of applications, including antibody labeling.
7/21.