Lumit® Immunoassay Detection Reagents Technical Manual
Instructions for Use of Product(s)
VB2010, VB2020, VB2030, VB4050, VB4060
Literature # TM614
The Lumit® Immunoassay is based on NanoLuc® Binary Technology (NanoBiT). NanoBiT is a structural complementation system designed for biomolecular interaction studies. The NanoBiT® system is composed of two subunits, Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide), that have been optimized for stability and minimal self-association due to weak affinity (190μM). In Lumit® Immunoassays, antibodies are chemically labeled with SmBiT and LgBiT subunits. Upon binding of the labeled antibodies to an analyte, the subunits come into close proximity, reassemble into a functional luciferase enzyme and generate a luminescent signal in the presence of substrate. Other immunoassay formats, including competition and indirect immunoassays, can also be developed using this technology.
The Lumit® Immunoassay Labeling Kit and Lumit® Immunoassay Detection Reagents enable you to develop Lumit® Immunoassays customized to your specific application. The following protocol is for use with Lumit® Immunoassay Detection Reagents. For the Lumit® Immunoassay Labeling Kit, refer to the Lumit® Immunoassay Labeling Kit Technical Manual, #TM602.
Summary of Changes
The following changes were made to the 2/24 revision of this document:
1. Corrected the name of Immunoassay Buffer A in Section 3.A.
2. Updated Lumit™ to Lumit®.
3. Changed font and cover image.
4. Updated patent statements.
5. Made minor text updates.
Revised 2/24.