PowerPlex® 18D System for Use on the Applied Biosystems® Genetic Analyzers Technical Manual
Instructions for Use of Product(s)
DC1802, DC1808
Literature # TMD031
The PowerPlex® 18D System allows co-amplification and four-color fluorescent detection of eighteen loci, including D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Amelogenin, vWA, D8S1179, TPOX, FGA, D19S433 and D2S1338. The PowerPlex® 18D System provides all materials necessary to amplify STR regions of genomic DNA, including a hot-start, nonproofreading thermostable DNA polymerase. The system is optimized for analysis of common database samples, such as unwashed FTA® card punches (i.e., direct amplification). Additionally, thermal cycling time is reduced from previous PowerPlex® protocols. The PowerPlex® 18D System is compatible with the Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
Summary of Changes
The following changes were made to the 10/21 revision of this document:
1. Added ProFlex® PCR System.
2. Updated Section 6.L.
3. Revised Section 7.
4. Updated Table 5.
5. Incorporated other general updates.
6. Updated cover page.
Revised 10/21.