PowerPlex® Fusion System for Use on the Spectrum CE System Technical Manual
Instructions for Use of Product(s)
DC2402, DC2408
Literature # TMD054
The PowerPlex® Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use. This five-color system allows co-amplification and fluorescent detection of the 20 autosomal loci in the expanded CODIS core loci (CSF1PO, FGA, TH01, TPOX, vWA, D1S1656, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11 and D22S1045) as well as Amelogenin and DYS391 for gender determination. The Penta D and Penta E loci are also included to increase discrimination and allow searching of databases that include profiles with these loci. This extended panel of STR markers is intended to satisfy both CODIS and ESS recommendations.
All necessary materials are provided to amplify STR regions of human genomic DNA, including a hot-start thermostable DNA polymerase. This manual contains a protocol to separate amplified products and detect separated material on the Spectrum CE System.
Created 7/22.