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We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

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Why Does Denatured DNA Absorb More Ultraviolet Light Than Double-Stranded DNA?

Hyperchromic Shift

The phenomenon of UV absorbance increasing as DNA is denatured is known as the hyperchromic shift. The purine and pyrimidine bases in DNA strongly absorb ultraviolet light. Double-stranded DNA absorbs less strongly than denatured DNA due to the stacking interactions between the bases. Single deoxynucleotides absorb more strongly than denatured DNA(1).

References

  1. Alexander, R.R. and Griffiths, J.M. (1993) Basic Biochemical Methods, 2nd Edition. J.M. Wiley and Sons, New York.
Hyperchromic Shift References

Learn more about Fluorometers

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