Limited Functionality Warning

We are currently updating our network. During this time, certain functionality may be unavailable, including online orders. We apologize for any inconvenience this may cause you.

Please contact Customer Service with any questions or comments.
Phone: (608) 274-4330
Toll-Free Phone: (800) 356-9526
Email: custserv@promega.com
Hours: 7am – 6pm, CST, Monday-Friday

Success

Thank you for verifying your email address.

Email Verification Error

There was an issue verifying your email address. Please try again or contact Customer Service.

Contact Customer Service

The Role of Cell-Free Rabbit Reticulocyte Expression Systems in Functional Genomics

Michele Arduengo, Elaine Schenborn and Robin Hurst
Promega Corporation

Abstract

In the broad area of functional genomics, that is the global characterization of proteins and their function, cell-free rabbit reticulocyte lysate (RRL) has been used extensively to elucidate the mechanisms of mammalian translation, cotranslational modifications, post-translational modifications and translocation of proteins. More recently, RRL has been used as the workhorse for manufacturing  the proteins engaged in interaction, selection and protein evolution studies from DNA or mRNA libraries either in microarray, display or in vitro expression cloning (IVEC) technologies. This chapter highlights recent functional proteomics applications that use cell-free mammalian RRL.

Related Products

Abstract Article Related Products Related Protocols Video Related Resources Frequently Asked Questions Related Articles
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
Estonia
Finland
France
/
Germany
/
Ireland
Iceland
Italy
/
Luxembourg
Netherlands
Norway
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Allow Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Allow Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Allow Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.