Notes: The authors used the Promega pSV-β-Galactosidase Control Vector, pGL3-Control and pGL3-Basic Vectors, Luciferase Assay Reagent, TNT^® Coupled Wheat Germ Extract Systems and TNT^® Coupled Reticulocyte Lysate Systems in their studies NF-κB. (0994)

Notes: Luciferase studies performed in HepG2 cells using constructs prepared in the pGL3 Basic Vector. The luciferase constructs were cotransfected with the pSV-beta-Galactosidase Control Vector to normalize for transfection efficiency. Luciferase activities were measured with the Luciferase Assay System. (0629)

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM^®-T Vector and sequenced. (1491)

Notes: Promega's pCAT^® Basic and pSV Beta-Galactosidase Vectors were used in this study. Beta-galactosidase activity was assayed with the Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer. Promega's pCAT Vectors have now been replaced by the next generation pCAT^® 3 Reporter Vectors. (0434)

Notes: The β-Galactosidase gene was excised from the pSV-β-Galactosidase Control Vector and used to make a construct for the production of a transgenic mouse line. The expressed β-Galactosidase protein was detected in mouse tissues by a enzymatic histochemical method. The mRNA of the enzyme was also measured in various tissues and normalized to GAPDH. Promoter activity was studied in COS cells. (0804)

Notes: In this study, regulation of the rat neuron-specific enolase gene was examined. The pCAT^®-Basic and pCAT^®-Control Vectors, and the pSV-β-Galactosidase Control Vector were used. Studies were performed in LTK-, Neuro2A, HeLa, and PC12 cells. The pCAT^® Vectors have now been replaced with the improved pCAT^®-3 Vector series. (1546)

Notes: The pSV-β-Galactosidase Control Vector was used to control transfections into Hep3B cells. (0976)

Notes: PolyA+ RNA was isolated from U87, K562, 293, HepB3, HeLa, NIH3T3 and lymphoblastiod total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for Northern analysis. Expression of CAT reporter constructs, derived from the pCAT^® Basic Vector, was studied in the U87, K562 and HeLa cells and normalized to control beta-galactosidase. (1698)