Notes: Luciferase mRNA was transcribed, and the mRNA was translated in a wheat germ extract system. Luminescence was measured by adding luciferin to the translation mixtures before incubation. The accumulation of full-sized luciferase correlated with the increase in light production, and translation arrest lead to immediate cessation of luciferase activity increase. The ribosome-bound protein had no detectable enzymatic activity, but enzymatic activity was shown a few seconds after luciferase was released from the ribosome. Renaturation of denatured luciferase under these conditions had a half-time of approx. 14 minutes, supporting the cotranslational folding hypothesis (peptides start to attain native structure while synthesizing on the ribosome). (0877)

Notes: This paper discusses the use of the TNT^® Coupled Reticulocyte Lysate System to express mitochondrial genes in the mammalian cytosol and check for protein expression. (0324)

Notes: The Wheat Germ Extract was used to generate proteins which, in combination with the Gel Shift Assay System, were used for gel shift assays. (0651)

Notes: Authors used the Riboprobe^® in vitro Transcription System to make RNA and translate it in the Wheat Germ Extract System to study myristlyation of the protein. (1170)

Notes: Protein products translated in vitro in Rabbit Reticulocyte Lysate and Wheat Germ Extract were used for gel shift assays. (0245)