Optimize your RNA workflow - hints and pitfalls of RT-qPCR
- RT-qPCR experimental workflow
- RNA/miRNA extraction
- RNA sample handling and protection
- Reverse Transcription
- Real-Time quantitative PCR
Summary
During this webinar you will learn more about optimizing your complete RNA workflow for reverse transcription real-time quantitative PCR (RT-qPCR) experiments. We will introduce you to the following steps of gene expression analysis: planning of the experiment, isolation of RNA from different sample types, reverse transcription as well as highly accurate qPCR quantification and data analysis. In addition, we provide you with helpful tips and tricks for your daily laboratory work to make your experiments more successful.
You will learn more about:
- RT-qPCR experimental workflow
- RNA/miRNA extraction
- RNA sample handling and protection
- Reverse Transcription
- Real-Time quantitative PCR
Speaker
Marcel Thiele
Field Application Specialist
Promega GmbH
Marcel was born in Berlin and graduated as BTA from the Lise-Meitner Schule. Furthermore, he received a Dipl.-Ing. in Medical Biotechnology from the Technical University Berlin. After working at the Fraunhofer IZI in Golm (2010 to 2011) he changed to the Max-Planck Institute for Molecular Genomics (MPIMG) where he focused on expression analysis of genes implicated in endoderm development. Marcel joined Promega in 2014. As a Field-Application Specialist, he is providing support to scientists in northeastern Germany. Being in constant exchange with customers and scientists from Promega’s Technical Service he could gain a deep understanding of common pitfalls of RNA analysis. This knowledge, spiked with his own experience, has been nicely combined into this RNA workflow webinar.