CloneWeaver® Workflow Purchasing Tool

Restriction Enzymes

Name	Description
4-CORE Buffer Pack	Open/Close	Add
Majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers
The 4-CORE Buffer Pack contains convenient aliquots of Promega Restriction Enzyme 10X Buffers A, B, C and D. The majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers. Visit Product Page »
AgeI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix
A/CCGG T T GGCC\A Visit Product Page »
BamHI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications
G/GATC C C CTAG\G Visit Product Page »
BclI	Open/Close	Add
Genome qualified to ensure optimal performance in genomic analysis applications
T/GATC A A CTAG\T Visit Product Page »
BglI	Open/Close	Add
Genome qualified to ensure optimal performance in genomic analysis applications
GCCN NNN/NGGC CGGN\NNN NCCG Visit Product Page »
DpnI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less
G(me)A/TC CT\(me)AG Visit Product Page »
EcoRI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Available at high concentration, containing 25,000 units of EcoRI at a concentration of 40–80u/μl
G/AATT C C TTAA\G Visit Product Page »
EcoRV	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
GAT/ATC CTA\TAG Visit Product Page »
HindIII	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
A/AGCT T T TCGA\A Visit Product Page »
MluI	Open/Close	Add
Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
A/CGCG T T GCGC\A Visit Product Page »
MULTI-CORE Buffer Pack	Open/Close	Add
Enables digestion of DNA with more than one enzyme in a single reaction Only modest adjustments in the amount of enzyme used will ensure complete multiple digestions
The MULTI-CORE Buffer Pack contains convenient aliquots of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE Buffer is formulated to provide simple buffering conditions for performing multiple digestions. Many Promega restriction enzymes have between 50% and 100% activity in reactions using MULTI-CORE Buffer. Visit Product Page »
NcoI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
C/CATG G G GTAC\C Visit Product Page »
NdeI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix
CA/TA TG GT AT\AC Visit Product Page »
NotI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
GC/GGCC GC CG CCGG\CG Visit Product Page »
PstI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, provides a higher level of quality control for enzymes used in cloning applications
C TGCA/G G\ACGT C Visit Product Page »
RsaI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less
GT/AC CA\TG Visit Product Page »
SacI	Open/Close	Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
G AGCT/C C\TCGA G Visit Product Page »
SalI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
G/TCGA C C AGCT\G Visit Product Page »
SgfI	Open/Close	Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
GCG AT/CGC CGC\TA GCG Visit Product Page »
SmaI	Open/Close	Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
CCC/GGG GGG\CCC Visit Product Page »
SpeI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
A/CTAG T T GATC\A Visit Product Page »
TaqI	Open/Close	Add
Restriction endonuclease from Thermus aquaticus YTI
T/CG A A GC\T Visit Product Page »
XbaI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
T/CTAG A A GATC\T Visit Product Page »
XhoI	Open/Close	Add
Capable of digesting DNA in 15 minutes or less Active and capable of digestion directly in GoTaq® Green Master Mix Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Genome qualified to ensure optimal performance in genomic analysis applications
C/TCGA G G AGCT\C Visit Product Page »

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